TY - JOUR
T1 - Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films
AU - Sanches-Silva, Ana
AU - Ribeiro, Tiago
AU - Albuquerque, Tânia G.
AU - Paseiro, Perfecto
AU - Sendón, Raquel
AU - De Quirós, Ana Bernaldo
AU - López-Cervantes, Jaime
AU - Sánchez-Machado, Dalia I.
AU - Valdez, Herlinda Soto
AU - Angulo, Inmaculada
AU - Aurrekoetxea, Goizane P.
AU - Costa, Helena S.
PY - 2012/3
Y1 - 2012/3
N2 - Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).
AB - Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).
KW - Chitosan
KW - Glucosamine
KW - Migration tests
KW - Shrimp by-products
KW - Ultra-high-pressure liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=84860171774&partnerID=8YFLogxK
U2 - 10.1002/jssc.201100855
DO - 10.1002/jssc.201100855
M3 - Article
C2 - 22517638
AN - SCOPUS:84860171774
SN - 1615-9306
VL - 35
SP - 633
EP - 640
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 5-6
ER -