Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films

Ana Sanches-Silva; Tiago Ribeiro; Tânia G. Albuquerque; Perfecto Paseiro; Raquel Sendón; Ana Bernaldo De Quirós; Jaime López-Cervantes; Dalia I. Sánchez-Machado; Herlinda Soto Valdez; Inmaculada Angulo; Goizane P. Aurrekoetxea; Helena S. Costa

doi:10.1002/jssc.201100855

Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films

Ana Sanches-Silva, Tiago Ribeiro, Tânia G. Albuquerque, Perfecto Paseiro, Raquel Sendón, Ana Bernaldo De Quirós, Jaime López-Cervantes, Dalia I. Sánchez-Machado, Herlinda Soto Valdez, Inmaculada Angulo, Goizane P. Aurrekoetxea, Helena S. Costa

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).

Original language	English
Pages (from-to)	633-640
Number of pages	8
Journal	Journal of Separation Science
Volume	35
Issue number	5-6
DOIs	https://doi.org/10.1002/jssc.201100855
Publication status	Published - Mar 2012
Externally published	Yes

Keywords

Chitosan
Glucosamine
Migration tests
Shrimp by-products
Ultra-high-pressure liquid chromatography

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10.1002/jssc.201100855

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Sanches-Silva, A., Ribeiro, T., Albuquerque, T. G., Paseiro, P., Sendón, R., De Quirós, A. B., López-Cervantes, J., Sánchez-Machado, D. I., Valdez, H. S., Angulo, I., Aurrekoetxea, G. P., & Costa, H. S. (2012). Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films. Journal of Separation Science, 35(5-6), 633-640. https://doi.org/10.1002/jssc.201100855

@article{c744cd3bc32e4e7eada019fd589f4090,

title = "Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films",

abstract = "Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).",

keywords = "Chitosan, Glucosamine, Migration tests, Shrimp by-products, Ultra-high-pressure liquid chromatography",

author = "Ana Sanches-Silva and Tiago Ribeiro and Albuquerque, {T{\^a}nia G.} and Perfecto Paseiro and Raquel Send{\'o}n and {De Quir{\'o}s}, {Ana Bernaldo} and Jaime L{\'o}pez-Cervantes and S{\'a}nchez-Machado, {Dalia I.} and Valdez, {Herlinda Soto} and Inmaculada Angulo and Aurrekoetxea, {Goizane P.} and Costa, {Helena S.}",

year = "2012",

month = mar,

doi = "10.1002/jssc.201100855",

language = "English",

volume = "35",

pages = "633--640",

journal = "Journal of Separation Science",

issn = "1615-9306",

publisher = "John Wiley and Sons Inc.",

number = "5-6",

}

Sanches-Silva, A, Ribeiro, T, Albuquerque, TG, Paseiro, P, Sendón, R, De Quirós, AB, López-Cervantes, J, Sánchez-Machado, DI, Valdez, HS, Angulo, I, Aurrekoetxea, GP & Costa, HS 2012, 'Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films', Journal of Separation Science, vol. 35, no. 5-6, pp. 633-640. https://doi.org/10.1002/jssc.201100855

TY - JOUR

T1 - Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films

AU - Sanches-Silva, Ana

AU - Ribeiro, Tiago

AU - Albuquerque, Tânia G.

AU - Paseiro, Perfecto

AU - Sendón, Raquel

AU - De Quirós, Ana Bernaldo

AU - López-Cervantes, Jaime

AU - Sánchez-Machado, Dalia I.

AU - Valdez, Herlinda Soto

AU - Angulo, Inmaculada

AU - Aurrekoetxea, Goizane P.

AU - Costa, Helena S.

PY - 2012/3

Y1 - 2012/3

N2 - Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).

AB - Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110°C, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).

KW - Chitosan

KW - Glucosamine

KW - Migration tests

KW - Shrimp by-products

KW - Ultra-high-pressure liquid chromatography

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U2 - 10.1002/jssc.201100855

DO - 10.1002/jssc.201100855

M3 - Article

C2 - 22517638

AN - SCOPUS:84860171774

SN - 1615-9306

VL - 35

SP - 633

EP - 640

JO - Journal of Separation Science

JF - Journal of Separation Science

IS - 5-6

ER -

Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films

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Keywords

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