Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino; Helena Barroso; Fátima Gonçalves; Sofia Marques Silva; Carlos Novo; Perpétua Gomes; Ricardo Camacho; Nuno Taveira

doi:10.1128/JCM.44.2.607-611.2006

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

José Maria Marcelino
, Helena Barroso
, Fátima Gonçalves
, Sofia Marques Silva
, Carlos Novo
, Perpétua Gomes
, Ricardo Camacho
, Nuno Taveira

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

Original language	English
Pages (from-to)	607-611
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1128/JCM.44.2.607-611.2006
Publication status	Published - Feb 2006

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10.1128/JCM.44.2.607-611.2006

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Marcelino, J. M., Barroso, H., Gonçalves, F., Silva, S. M., Novo, C., Gomes, P., Camacho, R., & Taveira, N. (2006). Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins. Journal of Clinical Microbiology, 44(2), 607-611. https://doi.org/10.1128/JCM.44.2.607-611.2006

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title = "Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins",

abstract = "A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100\% specificity. Clinical sensitivity was 100\% for rgp36 and 93.4\% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.",

author = "Marcelino, \{Jos{\'e} Maria\} and Helena Barroso and F{\'a}tima Gon{\c c}alves and Silva, \{Sofia Marques\} and Carlos Novo and Perp{\'e}tua Gomes and Ricardo Camacho and Nuno Taveira",

year = "2006",

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doi = "10.1128/JCM.44.2.607-611.2006",

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Marcelino, JM , Barroso, H, Gonçalves, F, Silva, SM, Novo, C, Gomes, P, Camacho, R & Taveira, N 2006, 'Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins', Journal of Clinical Microbiology, vol. 44, no. 2, pp. 607-611. https://doi.org/10.1128/JCM.44.2.607-611.2006

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins. / Marcelino, José Maria ; Barroso, Helena; Gonçalves, Fátima et al.
In: Journal of Clinical Microbiology, Vol. 44, No. 2, 02.2006, p. 607-611.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

AU - Marcelino, José Maria

AU - Barroso, Helena

AU - Gonçalves, Fátima

AU - Silva, Sofia Marques

AU - Novo, Carlos

AU - Gomes, Perpétua

AU - Camacho, Ricardo

AU - Taveira, Nuno

PY - 2006/2

Y1 - 2006/2

N2 - A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

AB - A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

UR - https://www.scopus.com/pages/publications/32344453650

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DO - 10.1128/JCM.44.2.607-611.2006

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AN - SCOPUS:32344453650

SN - 0095-1137

VL - 44

SP - 607

EP - 611

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Use of a new dual-antigen enzyme-linked immunosorbent assay to detect and characterize the human antibody response to the human immunodeficiency virus type 2 envelope gp125 and gp36 glycoproteins

Abstract

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