Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma

M. D.F. Santos; H. Vermeersch; J. P. Remon; M. Schelkens; P. De Backer; R. Ducatelle; F. Haesebrouck

doi:10.1016/0378-4347(96)00076-X

Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma

M. D.F. Santos, H. Vermeersch, J. P. Remon, M. Schelkens, P. De Backer, R. Ducatelle, F. Haesebrouck

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated. A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed. Linear calibration curves (r²>0.99) were obtained in water and plasma between 0 and 600 μg ml^-1. Good recoveries for doxycycline (>66%) and demeclocycline (>72%) were seen both in water and in plasma. The coefficient of variation was <9.86% for within-day reproducibility and <7.53% for the between-day reproducibility. The deviation between the mean value found and the true value was <14.5% (accuracy). The limit of detection was 0.1 μg ml^-1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at -20°C (recovery >91%).

Original language	English
Pages (from-to)	301-308
Number of pages	8
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	682
Issue number	2
DOIs	https://doi.org/10.1016/0378-4347(96)00076-X
Publication status	Published - 12 Jul 1996
Externally published	Yes

Keywords

Doxycycline

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10.1016/0378-4347(96)00076-X

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Santos, M. D. F., Vermeersch, H., Remon, J. P., Schelkens, M., De Backer, P., Ducatelle, R., & Haesebrouck, F. (1996). Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 682(2), 301-308. https://doi.org/10.1016/0378-4347(96)00076-X

@article{46c2d6cd020d44ef85aad2c27f718612,

title = "Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma",

abstract = "A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated. A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed. Linear calibration curves (r2>0.99) were obtained in water and plasma between 0 and 600 μg ml-1. Good recoveries for doxycycline (>66\%) and demeclocycline (>72\%) were seen both in water and in plasma. The coefficient of variation was <9.86\% for within-day reproducibility and <7.53\% for the between-day reproducibility. The deviation between the mean value found and the true value was <14.5\% (accuracy). The limit of detection was 0.1 μg ml-1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at -20°C (recovery >91\%).",

keywords = "Doxycycline",

author = "Santos, \{M. D.F.\} and H. Vermeersch and Remon, \{J. P.\} and M. Schelkens and \{De Backer\}, P. and R. Ducatelle and F. Haesebrouck",

note = "Funding Information: This study was performed with the financial support of the Flemish Institute for the promotion of Scientific-Technological Research in Industry (IWT).",

year = "1996",

month = jul,

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doi = "10.1016/0378-4347(96)00076-X",

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Santos, MDF, Vermeersch, H, Remon, JP, Schelkens, M, De Backer, P, Ducatelle, R & Haesebrouck, F 1996, 'Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 682, no. 2, pp. 301-308. https://doi.org/10.1016/0378-4347(96)00076-X

Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma. / Santos, M. D.F.; Vermeersch, H.; Remon, J. P. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 682, No. 2, 12.07.1996, p. 301-308.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma

AU - Santos, M. D.F.

AU - Vermeersch, H.

AU - Remon, J. P.

AU - Schelkens, M.

AU - De Backer, P.

AU - Ducatelle, R.

AU - Haesebrouck, F.

N1 - Funding Information: This study was performed with the financial support of the Flemish Institute for the promotion of Scientific-Technological Research in Industry (IWT).

PY - 1996/7/12

Y1 - 1996/7/12

N2 - A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated. A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed. Linear calibration curves (r2>0.99) were obtained in water and plasma between 0 and 600 μg ml-1. Good recoveries for doxycycline (>66%) and demeclocycline (>72%) were seen both in water and in plasma. The coefficient of variation was <9.86% for within-day reproducibility and <7.53% for the between-day reproducibility. The deviation between the mean value found and the true value was <14.5% (accuracy). The limit of detection was 0.1 μg ml-1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at -20°C (recovery >91%).

AB - A high-performance liquid chromatographic method for the analysis of doxycycline in turkey plasma samples using demeclocycline hydrochloride as the internal standard was developed, optimized and validated. A one-step extraction procedure and an isocratic HPLC method with UV detection were used. No interferences with endogenous compounds or with the anticoagulant were observed. Linear calibration curves (r2>0.99) were obtained in water and plasma between 0 and 600 μg ml-1. Good recoveries for doxycycline (>66%) and demeclocycline (>72%) were seen both in water and in plasma. The coefficient of variation was <9.86% for within-day reproducibility and <7.53% for the between-day reproducibility. The deviation between the mean value found and the true value was <14.5% (accuracy). The limit of detection was 0.1 μg ml-1 in plasma samples. A good stability of doxycycline was observed in water and in plasma samples after storage for six months at -20°C (recovery >91%).

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SN - 1572-6495

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 2

ER -

Validation of a high-performance liquid chromatographic method for the determination of doxycycline in turkey plasma

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