Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division

Cheok Man Chow; Andrew Georgiou; Henrietta Szutorisz; Alexandra Maia e Silva; Ana Pombo; Isabel Barahona; Elise Dargelos; Claudia Canzonetta; Niall Dillon

doi:10.1038/sj.embor.7400366

Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division

Cheok Man Chow, Andrew Georgiou, Henrietta Szutorisz, Alexandra Maia e Silva, Ana Pombo, Isabel Barahona, Elise Dargelos, Claudia Canzonetta, Niall Dillon

Research output: Contribution to journal › Article › peer-review

140 Citations (Scopus)

Abstract

Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.

Original language	English
Pages (from-to)	354-360
Number of pages	7
Journal	EMBO Reports
Volume	6
Issue number	4
DOIs	https://doi.org/10.1038/sj.embor.7400366
Publication status	Published - Apr 2005

Keywords

Epigenetic memory
Pre-B cell
Transcription

Access to Document

10.1038/sj.embor.7400366

Cite this

@article{b7fd307f184944be8e2b681624602069,

title = "Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division",

abstract = "Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.",

keywords = "Epigenetic memory, Pre-B cell, Transcription",

author = "Chow, {Cheok Man} and Andrew Georgiou and Henrietta Szutorisz and {Maia e Silva}, Alexandra and Ana Pombo and Isabel Barahona and Elise Dargelos and Claudia Canzonetta and Niall Dillon",

year = "2005",

month = apr,

doi = "10.1038/sj.embor.7400366",

language = "English",

volume = "6",

pages = "354--360",

journal = "EMBO Reports",

issn = "1469-221X",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division

AU - Chow, Cheok Man

AU - Georgiou, Andrew

AU - Szutorisz, Henrietta

AU - Maia e Silva, Alexandra

AU - Pombo, Ana

AU - Barahona, Isabel

AU - Dargelos, Elise

AU - Canzonetta, Claudia

AU - Dillon, Niall

PY - 2005/4

Y1 - 2005/4

N2 - Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.

AB - Variant histone H3.3 is incorporated into nucleosomes by a mechanism that does not require DNA replication and has also been implicated as a potential mediator of epigenetic memory of active transcriptional states. In this study, we have used chromatin immunoprecipitation analysis to show that H3.3 is found mainly at the promoters of transcriptionally active genes. We also show that H3.3 combines with H3 acetylation and K4 methylation to form a stable mark that persists during mitosis. Our results suggest that H3.3 is deposited principally through the action of chromatin-remodelling complexes associated with transcriptional initiation, with deposition mediated by RNA polymerase II elongation having only a minor role.

KW - Epigenetic memory

KW - Pre-B cell

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=17644369665&partnerID=8YFLogxK

U2 - 10.1038/sj.embor.7400366

DO - 10.1038/sj.embor.7400366

M3 - Article

C2 - 15776021

AN - SCOPUS:17644369665

SN - 1469-221X

VL - 6

SP - 354

EP - 360

JO - EMBO Reports

JF - EMBO Reports

IS - 4

ER -

Variant histone H3.3 marks promoters of transcriptionally active genes during mammalian cell division

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this