A fast and reliable method for GHB quantitation in whole blood by GC-MS/MS (TQD) for forensic purposes

André L. Castro, Sónia Tarelho, Mário Dias, Flávio Reis, Helena M. Teixeira

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Resumo

Gamma-hydroxybutyric acid (GHB) is an endogenous compound with a story of clinical use since the 1960s. However, due to its secondary effects, it has become a controlled substance, entering the illicit market. A fully validated, sensitive and reproducible method for the quantification of GHB by methanolic precipitation and GC-MS/MS (TQD) in whole blood is presented. Using 100μL of whole blood, obtained results included a LOD and LLOQ of 0.1mg/L and a recovery of 86% in a working range between 0.1 and 100mg/L. This method is sensitive and specific to detect the presence of GHB in small amounts of whole blood (both ante-mortem or post-mortem), and is, to the authors' knowledge, the first GC-MS-MS TQD method that uses different precursor ions and product ions for the identification of GHB and GHB-D6 (internal standard). Hence, this method may be especially useful for the study of endogenous values in this biological sample.

Idioma original???core.languages.en_GB???
Páginas (de-até)139-144
Número de páginas6
RevistaJournal of Pharmaceutical and Biomedical Analysis
Volume119
DOIs
Estado da publicação???researchoutput.status.published??? - 5 fev. 2016
Publicado externamenteSim

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