A new method for the HPLC analysis of uncflerivatized aminoacids with Evaporative Light-Scattering Detection

H. J. Chaves-das-Neves; Zilda Braga-Morais

A new method for the HPLC analysis of uncflerivatized aminoacids with Evaporative Light-Scattering Detection

H. J. Chaves-das-Neves, Zilda Braga-Morais

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A new HPLC method for separation and quantitation of 19 protein amino acids is described. No pre- or post-column derivatization is necessary. Separation is achieved on a mixed reversed-phase ion-exchange column with continuous monitoring of the column eluent by an Evaporative Light-Scattering Detection device (ELSD). Separation of 19 protein amino acids is obtained in a single 60 minutes run. A linear log-log relationship between integrated peak area vs. analyte mass is observed in a range of 50 to 1.330 ng with a detection limit of 10-20 ng and mean recoveries of 94%. Quantitative determinations can be made with a single calibration function for all the amino acids.

Idioma original	???core.languages.en_GB???
Páginas (de-até)	98-101
Número de páginas	4
Revista	Anales de Quimica
Volume	93
Número de emissão	2
Estado da publicação	???researchoutput.status.published??? - 1997
Publicado externamente	Sim

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@article{25569425070642c0b8f9224c257d3cd3,

title = "A new method for the HPLC analysis of uncflerivatized aminoacids with Evaporative Light-Scattering Detection",

abstract = "A new HPLC method for separation and quantitation of 19 protein amino acids is described. No pre- or post-column derivatization is necessary. Separation is achieved on a mixed reversed-phase ion-exchange column with continuous monitoring of the column eluent by an Evaporative Light-Scattering Detection device (ELSD). Separation of 19 protein amino acids is obtained in a single 60 minutes run. A linear log-log relationship between integrated peak area vs. analyte mass is observed in a range of 50 to 1.330 ng with a detection limit of 10-20 ng and mean recoveries of 94\%. Quantitative determinations can be made with a single calibration function for all the amino acids.",

keywords = "Aminoacids, Evaporative light-scattering detection (ELSD), HPLC",

author = "Chaves-das-Neves, \{H. J.\} and Zilda Braga-Morais",

year = "1997",

language = "English",

volume = "93",

pages = "98--101",

journal = "Anales de Quimica",

issn = "1130-2283",

publisher = "Springer-Verlag Iberica S.A.",

number = "2",

}

TY - JOUR

T1 - A new method for the HPLC analysis of uncflerivatized aminoacids with Evaporative Light-Scattering Detection

AU - Chaves-das-Neves, H. J.

AU - Braga-Morais, Zilda

PY - 1997

Y1 - 1997

N2 - A new HPLC method for separation and quantitation of 19 protein amino acids is described. No pre- or post-column derivatization is necessary. Separation is achieved on a mixed reversed-phase ion-exchange column with continuous monitoring of the column eluent by an Evaporative Light-Scattering Detection device (ELSD). Separation of 19 protein amino acids is obtained in a single 60 minutes run. A linear log-log relationship between integrated peak area vs. analyte mass is observed in a range of 50 to 1.330 ng with a detection limit of 10-20 ng and mean recoveries of 94%. Quantitative determinations can be made with a single calibration function for all the amino acids.

AB - A new HPLC method for separation and quantitation of 19 protein amino acids is described. No pre- or post-column derivatization is necessary. Separation is achieved on a mixed reversed-phase ion-exchange column with continuous monitoring of the column eluent by an Evaporative Light-Scattering Detection device (ELSD). Separation of 19 protein amino acids is obtained in a single 60 minutes run. A linear log-log relationship between integrated peak area vs. analyte mass is observed in a range of 50 to 1.330 ng with a detection limit of 10-20 ng and mean recoveries of 94%. Quantitative determinations can be made with a single calibration function for all the amino acids.

KW - Aminoacids

KW - Evaporative light-scattering detection (ELSD)

KW - HPLC

UR - https://www.scopus.com/pages/publications/0002645043

M3 - Article

AN - SCOPUS:0002645043

SN - 1130-2283

VL - 93

SP - 98

EP - 101

JO - Anales de Quimica

JF - Anales de Quimica

IS - 2

ER -

A new method for the HPLC analysis of uncflerivatized aminoacids with Evaporative Light-Scattering Detection

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