Cloning of two β-tubulin related genes in tetrahymena pyriformis

Isabel Barahona; Lisete Galego; Michel Jacquet; Claudina Rodrigues-Pousada

doi:10.1016/0014-5793(85)80377-X

Cloning of two β-tubulin related genes in tetrahymena pyriformis

Isabel Barahona, Lisete Galego, Michel Jacquet, Claudina Rodrigues-Pousada

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Resumo

Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT₁ and βTT₂, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

Idioma original	???core.languages.en_GB???
Páginas (de-até)	227-232
Número de páginas	6
Revista	FEBS Letters
Volume	188
Número de emissão	2
DOIs	https://doi.org/10.1016/0014-5793(85)80377-X
Estado da publicação	???researchoutput.status.published??? - 2 set. 1985
Publicado externamente	Sim

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@article{aac812021d864d2496ecf09e2aabc62f,

title = "Cloning of two β-tubulin related genes in tetrahymena pyriformis",

abstract = "Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.",

keywords = "Gene organization, Tetrahymena pyriformis, β-Tubulin",

author = "Isabel Barahona and Lisete Galego and Michel Jacquet and Claudina Rodrigues-Pousada",

note = "Funding Information: and to Mrs Ana Barreiros for her skilful technical assistance.T his work was supported by grants from the Centre National de la RechercheS cien-tifique and CalousteG ulbenkianF oundation. I.B. thanks the French Governmentf or a fellowship.",

year = "1985",

month = sep,

day = "2",

doi = "10.1016/0014-5793(85)80377-X",

language = "English",

volume = "188",

pages = "227--232",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

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TY - JOUR

T1 - Cloning of two β-tubulin related genes in tetrahymena pyriformis

AU - Barahona, Isabel

AU - Galego, Lisete

AU - Jacquet, Michel

AU - Rodrigues-Pousada, Claudina

N1 - Funding Information: and to Mrs Ana Barreiros for her skilful technical assistance.T his work was supported by grants from the Centre National de la RechercheS cien-tifique and CalousteG ulbenkianF oundation. I.B. thanks the French Governmentf or a fellowship.

PY - 1985/9/2

Y1 - 1985/9/2

N2 - Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

AB - Genomic clones containing β-tubulin sequences were isolated from a pBR322 genomic library of Tetrahymena pyriformis. Each clone has been mapped and the identity of the tubulin-related genes has been established by cross-hybridization with a cDNA containing a complete β-tubulin coding region from Chlamydomonas and by the positive hybrid-selected translation assay. The respective tubulin-related genes, βTT1 and βTT2, differ from each other although they contain a homologous region which is also homologous with a heterologous probe. Tetrahymena β-tubulin-related genes are physically linked and clustered in the genome.

KW - Gene organization

KW - Tetrahymena pyriformis

KW - β-Tubulin

UR - http://www.scopus.com/inward/record.url?scp=0022219693&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(85)80377-X

DO - 10.1016/0014-5793(85)80377-X

M3 - Article

AN - SCOPUS:0022219693

SN - 0014-5793

VL - 188

SP - 227

EP - 232

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Cloning of two β-tubulin related genes in tetrahymena pyriformis

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