Construction of disposable biosensors for nitrite monitoring in food and beverages

Tiago Monteiro, Célia M. Silveira, Elena Jubete, M. Gabriela Almeida

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Resumo

Nitrite salts are regularly used as fertilizers and food preservatives. Excessive levels have raised awareness from public regulation agencies, since nitrite can have an adverse impact on public health and on ecological systems. Therefore, it is important to develop sensitive assays to monitor this ion in foodstuff products and waters. This work presents a nitrite biosensor based on a highly selective nitrite reducing enzyme incorporated on carbon ink based screen-printed electrodes. The resulting device enabled the quantification of nitrite in different samples (milk, water) in a straightforward way and with small error (1-6%). The sensitivity of the biosensor towards nitrite reduction under optimized conditions was 0.55 A M-1 cm-2 with a linear response in the range 0.7-370 jM.Grant: This work was supported byCiiEM - Centro de Investigaçao Interdisciplinar Egas Moniz, and Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO, which is financed by national funds from FCT/MEC (UID/Multi/ 04378/2013) and co-financed by the ERDF under the PT2020 Partnership Agreement (POCI-01-0145-FEDER-007728). CM. Silveira and T. Monteiro acknowledge Fundaçao para a Ciencia e Tecnologia the fellowships SFRH/BPD/79566/2011 and PD/BD/109687/2015, respectively.

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Título da publicação do anfitrião2nd IMEKOFOODS Conference
Subtítulo da publicação do anfitriãoPromoting Objective and Measurable Food Quality and Safety
EditoraUniversity of Sannio
Páginas219-223
Número de páginas5
ISBN (eletrónico)9781510844957
Estado da publicação???researchoutput.status.published??? - 2016
Evento2nd IMEKOFOODS Conference: Promoting Objective and Measurable Food Quality and Safety 2016 - Benevento
Duração: 2 out. 20165 out. 2016

Série de publicação

Nome2nd IMEKOFOODS Conference: Promoting Objective and Measurable Food Quality and Safety

???event.eventtypes.event.conference???

???event.eventtypes.event.conference???2nd IMEKOFOODS Conference: Promoting Objective and Measurable Food Quality and Safety 2016
País/TerritórioItaly
CidadeBenevento
Período2/10/165/10/16

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