Exploring a new jellyfish collagen in the production of microparticles for protein delivery

M. Teresa Calejo, António J. Almeida, Ana I. Fernandes

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Resumo

A microparticulate protein delivery system was developed using collagen, from the medusa Catostylus tagi, as a polymeric matrix. Collagen microparticles (CMPs) were produced by an emulsification-gelation-solvent extraction method and a high loading efficiency was found for the entrapment of lysozyme and -lactalbumin. CMPs were cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The uncross-linked CMPs were spherical, rough-surfaced, presenting an estimated median size of 28m by laser diffraction. Upon cross-linking, particle size (9.5m) and size distribution were reduced. CMPs showed a moderate hydrophobic behaviour and a positive surface charge. Cross-linking also resulted in greater stability in water, allowing a slow release, as shown by in vitro experiments. The assessment of lysozyme's biological activity showed that the protein remained active throughout the encapsulation and cross-linking processes. In summary, the work herein described shows the potential use of a marine collagen in the production of microparticles for the controlled release of therapeutic proteins.

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Páginas (de-até)520-531
Número de páginas12
RevistaJournal of Microencapsulation
Volume29
Número de emissão6
DOIs
Estado da publicação???researchoutput.status.published??? - set. 2012

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