Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

J. C.G. Caldeira; J. M.S. Cabral

Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

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Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min^-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

Idioma original	???core.languages.en_GB???
Páginas (de-até)	271-278
Número de páginas	8
Revista	Bioseparation
Volume	4
Número de emissão	4
Estado da publicação	???researchoutput.status.published??? - ago. 1994
Publicado externamente	Sim

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title = "Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells",

abstract = "Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.",

author = "Caldeira, {J. C.G.} and Cabral, {J. M.S.}",

year = "1994",

month = aug,

language = "English",

volume = "4",

pages = "271--278",

journal = "Bioseparation",

issn = "0923-179X",

publisher = "Kluwer Academic Publishers",

number = "4",

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T1 - Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

AU - Caldeira, J. C.G.

AU - Cabral, J. M.S.

PY - 1994/8

Y1 - 1994/8

N2 - Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

AB - Extraction of a steroid 1,2 dehydrogenase was achieved by sonication after stabilization by the addition of monohydrated sodium glutamate (1.5 M). A specific first order rate of enzyme release of 0.035 min-1 was obtained. Studies were carried out on the following sonication parameters: power of sonication, cell concentration and container volume. A total recirculating system appears to be an advantageous approach to process larger volumes.

UR - http://www.scopus.com/inward/record.url?scp=0028485766&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0028485766

SN - 0923-179X

VL - 4

SP - 271

EP - 278

JO - Bioseparation

JF - Bioseparation

IS - 4

ER -

Extraction of a steroid 1,2 dehydrogenase by sonication of Arthrobacter simplex cells

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