TY - JOUR
T1 - Genetic characterization of an insect-specific flavivirus isolated from Culex theileri mosquitoes collected in southern Portugal
AU - Parreira, Ricardo
AU - Cook, Shelley
AU - Lopes, Ângela
AU - de Matos, António Pedro
AU - de Almeida, António Paulo Gouveia
AU - Piedade, João
AU - Esteves, Aida
N1 - Funding Information:
We would like to thank Dr. Anna-Bella Failloux (Unité de Génétique Moléculaire des Bunyavirus, Institut Pasteur, Paris, France) for her kind gift of C6/36 cells, and Dr. V. Stollar (Department of Molecular Genetics, Microbiology & Immunology, UMDNJ Robert Wood Johnson Medical School, New Brunswick, New Jersey, USA) for CFAV. We also acknowledge Sónia Costa and Ferdinando Bernardino de Freitas for technical assistance. This work was partially by Fundação para a Ciência e a Tecnologia through UPMM funds.
PY - 2012/8
Y1 - 2012/8
N2 - We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs. The CTFV genome encodes a single polyprotein with 3357 residues showing all the features expected for those of flaviviruses. Phylogenetic analyses based on all ISF sequences available to date, place CTFV among Culex-associated flaviviruses, grouping with recently published NS5 partial sequences documented from mosquitoes collected in the Iberian Peninsula, and with Quang Binh virus (isolated in Vietnam) as a close relative. No CTFV sequences were found integrated in their host's genome using a range of specific PCR primers designed to the prM/E, NS3, and NS5 region.
AB - We describe the full genetic characterization of an insect-specific flavivirus (ISF) from Culex theileri (Theobald) mosquitoes collected in Portugal. This represents the first isolation and full characterization of an ISF from Portuguese mosquitoes. The virus, designated CTFV, for Culex theileri flavivirus, was isolated in the C6/36 Stegomyia albopicta (=Aedes albopictus) cell line, and failed to replicate in vertebrate (Vero) cells in common with other ISFs. The CTFV genome encodes a single polyprotein with 3357 residues showing all the features expected for those of flaviviruses. Phylogenetic analyses based on all ISF sequences available to date, place CTFV among Culex-associated flaviviruses, grouping with recently published NS5 partial sequences documented from mosquitoes collected in the Iberian Peninsula, and with Quang Binh virus (isolated in Vietnam) as a close relative. No CTFV sequences were found integrated in their host's genome using a range of specific PCR primers designed to the prM/E, NS3, and NS5 region.
KW - Complete genomic sequence
KW - Iberian Peninsula
KW - Insect-specific flavivirus
KW - Phylogenetic analysis
KW - Portugal
UR - https://www.scopus.com/pages/publications/84863876738
U2 - 10.1016/j.virusres.2012.04.010
DO - 10.1016/j.virusres.2012.04.010
M3 - Article
C2 - 22579596
AN - SCOPUS:84863876738
SN - 0168-1702
VL - 167
SP - 152
EP - 161
JO - Virus Research
JF - Virus Research
IS - 2
ER -