@article{04d6572e1ef9476db49495b0bef6a504,
title = "How to dissect viral infections and their interplay with the host-proteome by immunoaffinity and mass spectrometry: A tutorial",
abstract = "The capabilities of bioanalytical mass spectrometry to (i) detect and differentiate viruses at the peptide level whilst maintaining high sample throughput and (ii) to provide diagnosis and prognosis for infected patients are presented as a tutorial in this work to aid analytical chemists and physicians to gain insights into the possibilities offered by current high-resolution mass spectrometry technology and bioinformatics. From (i) sampling to sample treatment; (ii) Matrix-Assisted Laser Desorption Ionization- to Electrospray Ionization -based mass spectrometry; and (iii) from clustering to peptide sequencing; a detailed step-by-step guide is provided and exemplified using SARS-CoV-2 Spike Y839 variant and the variant of concern SARS-CoV-2 Alpha (B.1.1.7 lineage), Influenza B, and Influenza A subtypes AH1N1pdm09 and AH3N2.",
keywords = "Immuno-mass spectrometry, LC-ESI-Q-TOF-MS/MS, MALDI-TOF-MS, Viral detection",
author = "Santos, {Hugo M.} and Carvalho, {Lu{\'i}s B.} and Carlos Lodeiro and Gon{\c c}alo Martins and Gomes, {In{\^e}s L.} and Antunes, {Wilson D.T.} and Vanessa Correia and Almeida-Santos, {Maria M.} and Helena Rebelo-de-Andrade and Matos, {Ant{\'o}nio P.A.} and Capelo, {J. L.}",
note = "Funding Information: PROTEOMASS Scientific Society is acknowledged by the funding provided to the Laboratory for Biological Mass Spectrometry Isabel Moura (#PM001/2019 and #PM003/2016). H.M.S, L.B.C. C.L., G.M., and J.L.C. acknowledge the funding provided by the Associate Laboratory for Green Chemistry LAQV which is financed by national funds from FCT/MCTES, Funda{\c c}{\~a}o para a Ci{\^e}ncia e Tecnologiaand Minist{\'e}rio da Ci{\^e}ncia, Tecnologia e Ensino Superior, through the projects UIDB/50006/2020 and UIDP/50006/2020. H.M.S acknowledges the Associate Laboratory for Green Chemistry-LAQV (LA/P/0008/2020) funded by FCT/MCTES for his research contract. L.B.C., G.M. thank FCT/MCTES (Portugal) for their PhD grant (SFRH/BD/144222/2019) and (SFRH/BD/139384/2018), respectively. The authors acknowledge Dr. Miguel Fevereiro from INIAV for his valuable help and fruitful discussions. Funding Information: This work received financial support from PT national funds (FCT/MCTES) through the projects UIDB/50006/2020 and UIDP/50006/2020 and from PROTEOMASS Scientific Society through the projects #PM001/2019 and #PM003/2016. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2023",
month = mar,
doi = "10.1016/j.microc.2022.108323",
language = "English",
volume = "186",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",
}
