TY - JOUR
T1 - In vitro reassembly of the malolactic fermentation pathway of Leuconostoc oenos (Oenococcus oeni)
AU - Salema, Madalena
AU - Capucho, Inácia
AU - Poolman, Bert
AU - San Romão, M. V.
AU - Loureiro Dias, Maria C.
PY - 1996
Y1 - 1996
N2 - The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M.D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.
AB - The mechanism of metabolic energy generation by malolactic fermentation was studied with artificial membrane vesicles of Leuconostoc oenos (Oenococcus oeni). (Note that although L. oenos was recently reclassified as O. oeni [L. M. T. Dicks, F. Dellaglio, and M.D. Collins, Int. J. Syst. Bacteriol. 45:395-397, 1995], the old designation was kept in the present work.) Purified malolactic enzyme was entrapped in artificial membrane vesicles prepared from L. oenos cells able to transport L-malate. We show that the in vitro reconstituted system, including an electrogenic L-malate carrier and the decarboxylating malolactic enzyme, generated a proton motive force that was able to drive intravesicular accumulation of leucine.
UR - http://www.scopus.com/inward/record.url?scp=0029788139&partnerID=8YFLogxK
U2 - 10.1128/jb.178.18.5537-5539.1996
DO - 10.1128/jb.178.18.5537-5539.1996
M3 - Article
C2 - 8808948
AN - SCOPUS:0029788139
SN - 0021-9193
VL - 178
SP - 5537
EP - 5539
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 18
ER -