Klonirovanie fragmentov gomeobokssoderzhashchikh genov, ékspressiruiushchikhsia v khode regeneratsii planarii.

K. A. Luk'ianov, V. S. Tarabykin, V. K. Potapov, E. P. Bekman, S. A. Luk'ianov

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Resumo

The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.

Título traduzido da contribuiçãoThe cloning of fragments of homeobox genes expressed during planarian regeneration
Idioma original???core.languages.ru_RU???
Páginas (de-até)28-32
Número de páginas5
RevistaOntogenez
Volume25
Número de emissão6
Estado da publicação???researchoutput.status.published??? - nov. 1994

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