Resumo
The polymerase chain reaction with degenerate primers corresponding to the most conservative amino acids 16-21 (ELEKEF) and 49-54 (WFQNRR) of the Antennapedia class homeodomain was used for the amplification of cDNA from regenerating planarians (asexual race of Dugesia tigrina). A total of six new Antennapedia-like homeobox sequences, designated Dutarh-1-Dutarh-6 (Dugesia tigrina asexual race homeobox gene), were obtained. Their comparison with other homeobox genes using a "Genebee" software (the EMBL Data Library) showed that all sequences except Dutarh-6 belong to the Antennapedia class. Dutarh-6 is closely related to a recently described novel homeobox gene subfamily that includes mouse mesodermal homeobox genes Mox-1 and Mox-2 and rat homeobox gene Gax.
Título traduzido da contribuição | The cloning of fragments of homeobox genes expressed during planarian regeneration |
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Idioma original | ???core.languages.ru_RU??? |
Páginas (de-até) | 28-32 |
Número de páginas | 5 |
Revista | Ontogenez |
Volume | 25 |
Número de emissão | 6 |
Estado da publicação | ???researchoutput.status.published??? - nov. 1994 |