Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay

Perpétua Gomes; Nuno Costa Taveira; José Moniz Pereira; Francisco Antunes; M. O. Santos Ferreira; Maria Helena Lourenço

doi:10.1128/jcm.37.2.453-456.1999

Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay

Perpétua Gomes
, Nuno Costa Taveira
, José Moniz Pereira
, Francisco Antunes
, M. O. Santos Ferreira
, Maria Helena Lourenço

Resultado de pesquisa: ???type-name??? › ???researchoutput.researchoutputtypes.contributiontojournal.article??? › revisão de pares

29 Citações (Scopus)

Resumo

A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.

Idioma original	???core.languages.en_GB???
Páginas (de-até)	453-456
Número de páginas	4
Revista	Journal of Clinical Microbiology
Volume	37
Número de emissão	2
DOIs	https://doi.org/10.1128/jcm.37.2.453-456.1999
Estado da publicação	???researchoutput.status.published??? - 1999

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title = "Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay",

abstract = "A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.",

author = "Perp{\'e}tua Gomes and Taveira, \{Nuno Costa\} and \{Moniz Pereira\}, Jos{\'e} and Francisco Antunes and \{Santos Ferreira\}, \{M. O.\} and Louren{\c c}o, \{Maria Helena\}",

year = "1999",

doi = "10.1128/jcm.37.2.453-456.1999",

language = "English",

volume = "37",

pages = "453--456",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "2",

}

Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay. / Gomes, Perpétua ; Taveira, Nuno Costa; Moniz Pereira, José et al.
Em: Journal of Clinical Microbiology, Vol. 37, N.º 2, 1999, p. 453-456.

Resultado de pesquisa: ???type-name??? › ???researchoutput.researchoutputtypes.contributiontojournal.article??? › revisão de pares

TY - JOUR

T1 - Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay

AU - Gomes, Perpétua

AU - Taveira, Nuno Costa

AU - Moniz Pereira, José

AU - Antunes, Francisco

AU - Santos Ferreira, M. O.

AU - Lourenço, Maria Helena

PY - 1999

Y1 - 1999

N2 - A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.

AB - A new quantitative-competitive PCR-based human immunodeficiency virus type 2 (HIV-2) proviral DNA assay (QC-PCR) was developed and used to determine the provital load in HIV-2-infected individuals. Proviral load varied considerably, with means of 1,831 copies per 106 peripheral blood mononuclear cells for asymptomatic subjects (n = 19) and 2,587 for AIDS patients (n = 2). HIV-2 viral and provital loads also varied significantly over time in asymptomatic patients. These data suggest that a high level of virus replication occurs throughout the asymptomatic phase of HIV-2 infection.

UR - https://www.scopus.com/pages/publications/0345683492

U2 - 10.1128/jcm.37.2.453-456.1999

DO - 10.1128/jcm.37.2.453-456.1999

M3 - Article

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AN - SCOPUS:0345683492

SN - 0095-1137

VL - 37

SP - 453

EP - 456

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Quantitation of human immunodeficiency virus type 2 DNA in peripheral blood mononuclear cells by using a quantitative-competitive PCR assay

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