Resumo
In the present work, Ion Jelly films based on 1-ethyl-3-methylimidazolium ethylsulfate, with incorporated redox proteins and enzymes were deposited on carbon screen-printed electrodes (SPEs), and their electrochemical characterization was attained. Ion Jelly synthesis was carried out simultaneously with protein incorporation in its structure, followed by a maturation step under controlled atmosphere (4 days at 4 °C and water activity (aw) of 0.76). The electrochemical response of the material was characterized, and a sandwich-type fuel cell configuration was subsequently built, consisting of two SPEs containing two independent Ion Jelly discs in the middle; one disc incorporated Desulfovibrio gigas cytochrome c3 and [NiFe]-hydrogenase, while the other disc contained aldehyde oxidoreductase, constituting the biocathode and bioanode of the cell, respectively. Cell voltage increased with time in the presence of benzaldehyde, in agreement with a successful electronic pathway across the cell and the concomitant aldehyde oxidoreductase enzymatic activity. In the cathodic side assays, hydrogenase showed catalytic activity towards H+ reduction to H2.
Idioma original | ???core.languages.en_GB??? |
---|---|
Páginas (de-até) | 6546-6552 |
Número de páginas | 7 |
Revista | ChemistrySelect |
Volume | 1 |
Número de emissão | 20 |
DOIs | |
Estado da publicação | ???researchoutput.status.published??? - 1 dez. 2016 |
Publicado externamente | Sim |