Site of tubulin synthesis in Tetrahymena pyriformis

Isabel Barahona; Claudina Rodrigues-Pousada

doi:10.1016/0014-5793(83)80593-6

Site of tubulin synthesis in Tetrahymena pyriformis

Isabel Barahona
, Claudina Rodrigues-Pousada

Resultado de pesquisa: ???type-name??? › ???researchoutput.researchoutputtypes.contributiontojournal.article??? › revisão de pares

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Resumo

Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the ³⁵S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

Idioma original	???core.languages.en_GB???
Páginas (de-até)	271-275
Número de páginas	5
Revista	FEBS Letters
Volume	158
Número de emissão	2
DOIs	https://doi.org/10.1016/0014-5793(83)80593-6
Estado da publicação	???researchoutput.status.published??? - 25 jul. 1983
Publicado externamente	Sim

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title = "Site of tubulin synthesis in Tetrahymena pyriformis",

abstract = "Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.",

keywords = "Immunoprecipitation, In vitro protein synthesis, Membrane-bound and free polysomes, Tubulin",

author = "Isabel Barahona and Claudina Rodrigues-Pousada",

year = "1983",

month = jul,

day = "25",

doi = "10.1016/0014-5793(83)80593-6",

language = "English",

volume = "158",

pages = "271--275",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Site of tubulin synthesis in Tetrahymena pyriformis

AU - Barahona, Isabel

AU - Rodrigues-Pousada, Claudina

PY - 1983/7/25

Y1 - 1983/7/25

N2 - Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

AB - Free and membrane-bound polysomes were isolated from the protozoa Tetrahymena pyriformis, and the contribution of these two types of polysomes to tubulin synthesis were studied using immunoprecipitation of the 35S-translational products in a rabbit reticulocyte lysate. One-dimensional electrophoretic analysis shows that tubulin is synthesized by polyadenylated RNA isolated from free and membrane-bound polysomes. Non-polyadenylated RNAs of free polysomes are also able to direct tubulin synthesis. Two-dimensional electrophoretic analysis using O'Farrell's system confirms these results and also reveals the existence of the α- and β-tubulin subunits.

KW - Immunoprecipitation

KW - In vitro protein synthesis

KW - Membrane-bound and free polysomes

KW - Tubulin

UR - https://www.scopus.com/pages/publications/0021112364

U2 - 10.1016/0014-5793(83)80593-6

DO - 10.1016/0014-5793(83)80593-6

M3 - Article

C2 - 6192014

AN - SCOPUS:0021112364

SN - 0014-5793

VL - 158

SP - 271

EP - 275

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Site of tubulin synthesis in Tetrahymena pyriformis

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